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Catalog #
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Source:
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Lot #
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Size:
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AP7537a
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Rabbit
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SH030418G; SH030418H
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q13627
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RB3015/3016
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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85584 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DYRKA.
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Other Names:
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Dual-specificity tyrosine-phosphorylation regulated kinase 1A, Protein kinase minibrain homolog, MNBH, RP86, Dual specificity YAK1-related kinase
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Application Data:
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Western blot analysis of anti-DYRKA Antibody (N-term) (Cat.#AP7537a) in mouse liver tissue lysates (35ug/lane). DYRKA (arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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DYRK1A is a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome.
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Background References:
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- Guimera, J., et al., Genomics 57(3):407-418 (1999).
- Wang, J., et al., Biochem. Biophys. Res. Commun. 250(3):704-710 (1998).
- Becker, W., et al., J. Biol. Chem. 273(40):25893-25902 (1998).
- Dahmane, N., et al., Genomics 48(1):12-23 (1998).
- Chen, H., et al., Hum. Genet. 99(2):262-265 (1997).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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