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Catalog #
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Source:
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Lot #
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Size:
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AP7563c
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Rabbit
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SH080429L
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004337, P42574
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RB14696
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, FC, E
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H
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31608 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CASP3.
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Other Names:
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Caspase-3 [Precursor]; CASP-3; Apopain; Cysteine protease CPP32; Yama protein; CPP-32; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3
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Application Data:
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Western blot analysis of anti-CASP3 Pab (Cat.#AP7563c) in NCI-H460 cell line lysates (35ug/lane).CASP3 (arrow) was detected using the purified Pab (1:60 dilution). |
(LEFT) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 antibody (Center) (Cat.#AP7563c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT) Flow cytometric analysis of NCI-H460 cells using Caspase-3 (CASP3) Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Background:
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CASP3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
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Background References:
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- Wright,M.H.,Biochem. Biophys. Res. Commun. 369 (2), 478-484 (2008)
- Brown,E.T.,Radiat. Res. 169 (5), 595-601 (2008)
- Mustafa,T.,Virchows Arch. 452 (4), 449-456 (2008)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
| Flow cytometric | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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