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Catalog #
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Source:
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Lot #
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Size:
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AP7568b
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Rabbit
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SH080516C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004530, O43776
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RB14850
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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62943 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NARS.
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Other Names:
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Asparaginyl-tRNA synthetase, cytoplasmic; Asparagine--tRNA ligase; AsnRS; NARS; ASNS
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Application Data:
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Western blot analysis of anti-NARS Pab (Cat.#AP7568b) in Jurkat cell line lysates (35ug/lane).NARS (arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NARS antibody (C-term) (Cat.#AP7568b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase (NARS)is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
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Background References:
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- Lim,J., Cell 125 (4), 801-814 (2006)
- Lehner,B., Genome Res. 14 (7), 1315-1323 (2004)
- Beaulande,M., Nucleic Acids Res. 26 (2), 521-524 (1998)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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