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Catalog #
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Source:
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Lot #
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Size:
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AP7574c
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Rabbit
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SA070529C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005336, P08107
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RB10997
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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70052 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S525 of human HSPA1A.
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Other Names:
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Heat shock 70 kDa protein 1; HSP70.1; HSP70-1/HSP70-2
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Application Data:
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Western blot analysis of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (S525) (Cat.#AP7574c).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies). |
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Background:
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HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.
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Background References:
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- Mueller, T., et al., Transplantation 78(2):292-295 (2004).
- Fekete, A., et al., Pediatr. Res. 54(4):452-455 (2003).
- Broquet, A.H., et al., J. Biol. Chem. 278(24):21601-21606 (2003).
- Bruce, C.R., et al., Diabetes 52(9):2338-2345 (2003).
- Anwar, A., et al., J. Biol. Chem. 277(16):14060-14067 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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