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Catalog #
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Source:
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Lot #
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Size:
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AP7593b
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Rabbit
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SA080227AF
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005216, Q01094
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RB08111
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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46920 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S332 of human E2F1.
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Other Names:
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Transcription factor E2F1; E2F-1; Retinoblastoma binding protein 3; RBBP-3; PRB-binding protein E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; E2F1; RBBP3
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Application Data:
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Western blot analysis of anti-E2F1 Antibody (S332) (Cat.#AP7593b) in 293 cell line lysates (35ug/lane). E2F1 (arrow) was detected using the purified Pab (1:60dilution). |
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Background:
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E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
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Background References:
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- O'Donnell, K.A., et al., Nature 435(7043):839-843 (2005).
- Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).
- Joshi, B., et al., Oncogene 24(13):2204-2217 (2005).
- Saberwal, G., et al., Int. J. Hematol. 80(2):146-154 (2004).
- Chaussepied, M., et al., Mol. Cell 16(5):831-837 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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