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Catalog #
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Source:
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Lot #
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Size:
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AP7641d
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Rabbit
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SA080818AH
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P15473, NP_036720
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RB15353
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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31680 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S183 of human IGFBP3.
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Other Names:
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IBP3; BP-53; Insulin-like growth factor-binding protein 3; IGF-binding protein 3
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Application Data:
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Western blot analysis of IGFBP3(arrow) using rabbit polyclonal IGFBP3 Antibody (S183) (Cat.#AP7641d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with IGFBP gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IGFBP3 Antibody (S183) (Cat.#AP7641d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family with an IGFBP domain and a thyroglobulin type-I domain. This protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors.
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Background References:
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- Muzumdar,R.H., Diabetes 55 (10), 2788-2796 (2006)
- Novosyadlyy,R., Growth Horm. IGF Res. 15 (5), 313-323 (2005)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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