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Catalog #
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Source:
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Lot #
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Size:
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AP7656b
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Rabbit
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SH021107E
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P10721, Q99662
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RB1559
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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109864 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human KIT.
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Other Names:
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Mast/stem cell growth factor receptor; Proto-oncogene tyrosine-protein kinase Kit; SCFR; CD117 antigen
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Application Data:
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Western blot analysis of anti-KIT Pab (Cat. #AP7656b) in mouse pancreas lysate. KIT (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
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Background References:
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- Wardelmann, E., et al., Int. J. Cancer 106(6):887-895 (2003).
- Lennartsson, J., et al., Exp. Cell Res. 288(1):110-118 (2003).
- Sakuma, Y., et al., Cancer Sci 94(6):486-491 (2003).
- Araki, K., et al., Lung Cancer 40(2):173-180 (2003).
- Voytyuk, O., et al., J. Biol. Chem. 278(11):9159-9166 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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