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PDGFRB Antibody (C-term L1089)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP7667b Rabbit SH021106L; SH021107A 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml P09619 RB1555-1556 Rabbit Ig
Applications: Reactivity: MW (kDa):
IHC, E H 123967 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PDGFRB.
Other Names:
CD140b antigen; PDGF-R-beta
Application Data:
PDGFRB Antibody (C-term L1089)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
PDGFRB is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses the gene to that of the translocation, ETS, leukemia gene, results in chronic myelomonocytic leukemia.
Background References:
  1. Wu, H., et al., J. Biol. Chem. 278(42):40425-40428 (2003).
  2. Whiteman, E.L., et al., Endocrinology 144(9):3811-3820 (2003).
  3. Uren, A., et al., Oncogene 22(15):2334-2342 (2003).
  4. Steer, E.J., et al., Acta Haematol. 107(2):113-122 (2002).
  5. Kawagishi, J., et al., Genomics 30(2):224-232 (1995).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.