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Catalog #
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Source:
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Lot #
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Size:
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AP7694c
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Rabbit
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SA060330A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005148, P00519
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RB7355
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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122944 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ABL1.
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Other Names:
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Proto-oncogene tyrosine-protein kinase ABL1, p150, c-ABL
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Application Data:
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Western blot analysis of ABL1 Antibody (N-term H246) (Cat.#AP7694c) in Y79 cell line lysates (35ug/lane). ABL1(arrow) was detected using the purified Pab (1:60 dilution). |
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Background:
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The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.
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Background References:
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- Donaldson, L.W., et al., Proc. Natl. Acad. Sci. U.S.A. 99(22):14053-14058 (2002).
- Pisabarro, M.T., et al., J. Mol. Biol. 281(3):513-521 (1998).
- Nam, H.J., et al., Structure 4(9):1105-1114 (1996).
- Chissoe, S.L., et al., Genomics 27(1):67-82 (1995).
- Gosser, Y.Q., et al., Structure 3(10):1075-1086 (1995).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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