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Catalog #
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Source:
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Lot #
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Size:
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AP7743a
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Rabbit
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SG060531F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_003876, Q15078
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RB9405
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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IHC, E
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H
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34060 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CDK5R1.
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Other Names:
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Cyclin-dependent kinase 5 activator 1 [Precursor]; CDK5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; Tau protein kinase II 23 kDa subunit; p23; TPKII regulatory subunit; Cyclin-dependent kinase 5 activator 1, p35; Cyclin-dependent kinase 5 activator 1, p25; CDK5R1; CDK5R; NCK5A
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Application Data:
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Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5R1(p35) Antibody (N-term) (Cat.#AP7743a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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p35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.
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Background References:
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- Lin,S., FEBS Lett. 582 (8), 1197-1202 (2008)
- Sen,A., Neuroreport 18 (5), 511-516 (2007)
- Mitsios,N., Brain Pathol. 17 (1), 11-23 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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