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Catalog #
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Source:
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Lot #
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Size:
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AP7746a
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Rabbit
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SA080731AF
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_115584, Q9H6X2
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RB15324
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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62789 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y382 of human ANTXR1.
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Other Names:
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Anthrax toxin receptor 1 [Precursor]; Tumor endothelial marker 8; ANTXR1; ATR; TEM8
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Application Data:
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Western blot analysis of anti-ANTXR1 Antibody (Y382)(Cat.#AP7746a) in Hela cell line lysates (35ug/lane). ANTXR1 (arrow) was detected using the purified Pab (1:60dilution). |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANTXR1 Antibody (Y382) (Cat.#AP7746a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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ANTXR1 is a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. This protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes.
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Background References:
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- Werner,E., J. Biol. Chem. 281 (32), 23227-23236 (2006)
- Wei,W., Cell 124 (6), 1141-1154 (2006)
- Rainey,G.J., Proc. Natl. Acad. Sci. U.S.A. 102 (37), 13278-13283 (2005)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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