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Catalog #
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Source:
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Lot #
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Size:
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AP7756c
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Rabbit
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SH080410J
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000792, P62942
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RB14521
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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11951 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human FKBP12.
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Other Names:
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Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; EC 5.2.1.8; FK506-binding protein 1A; FKBP-1A; Rotamase; Immunophilin FKBP12; 12 kDa FKBP; FKBP-12; FKBP1A; FKBP1; FKBP12
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Application Data:
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Western blot analysis of FKBP12 (arrow) using rabbit polyclonal FKBP12 Antibody (Center) (Cat.#AP7756c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FKBP12 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FKBP12 antibody (Center) (Cat.#AP7756c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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FKBP12 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.
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Background References:
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- Gerard,M., J. Neurochem. 106 (1), 121-133 (2008)
- Shor,B., Cancer Res. 68 (8), 2934-2943 (2008)
- Jayaraman,T.,J. Biol. Chem. 267 (14), 9474-9477 (1992)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:100~500 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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