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Catalog #
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Source:
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Lot #
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Size:
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AP7773a
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Rabbit
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SA081017AF
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001341, Q9UER7
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RB6869
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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81373 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S495 of human DAXX.
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Other Names:
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Death domain-associated protein 6; Daxx; hDaxx; Fas death domain-associated protein; ETS1 associated protein 1; EAP1; DAXX; BING2; DAP6
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Application Data:
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Western blot analysis of anti-DAXX Antibody (S495) (Cat.#AP7773a) in K562 cell line lysates (35ug/lane). DAXX(arrow) was detected using the purified Pab. |
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Background:
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DAXX is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, this protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, this protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination.
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Background References:
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- Cantrell, S.R., et al., J. Virol. 79(12):7792-7802 (2005).
- Greger, J.G., et al., J. Virol. 79(8):4610-4618 (2005).
- Chang, C.C., et al., J. Biol. Chem. 280(11):10164-10173 (2005).
- Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).
- Gostissa, M., et al., J. Biol. Chem. 279(46):48013-48023 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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