|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP7779a
|
Rabbit
|
SH080530A
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_000600
|
RB14437
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
IHC, E
|
H
|
10535 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CXCL12.
|
|
Other Names:
|
|
Stromal cell-derived factor 1; SDF-1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; PBSF; hIRH; SDF-1-beta(3-72); SDF-1-alpha(3-67); CXCL12; SDF1; SDF1A; SDF1B
|
|
Application Data:
|
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CXCL12 antibody (N-term) (Cat.#AP7779a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
|
|
Background:
|
|
Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CXC chemokines are further subdivided into ELR and non-ELR types based on the presence or absence of a glu-leu-arg sequence adjacent and N terminal to the CXC motif. ELR types are chemotactic for neutrophils, while non-ELR types are chemotactic for lymphocytes.
Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF (see MIM 191160), or IL1 (see MIM 147760). The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.
|
|
Background References:
|
- Manetti,M., Ann. Rheum. Dis. (2008)
- Delgado,M.B., Eur. J. Immunol. 31 (3), 699-707 (2001)
- Oberlin,E., Nature 382 (6594), 833-835 (1996)
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Immunohistochemistry | 1:10~50 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
