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IRAK4 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP7805a Rabbit SH030211T 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_057207 RB2347 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 50899 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human IRAK4.
Other Names:
interleukin-1 receptor-associated kinase 4
Application Data:

Western blot analysis of anti-IRAK4 Pab (Cat. #AP7805a) in Jurkat cell lysate. IRAK4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.
Background References:
  1. Medvedev, A.E., et al., J. Exp. Med. 198(4):521-531 (2003).
  2. Jiang, Z., et al., J. Biol. Chem. 278(13):10952-10956 (2003).
  3. Picard, C., et al., Science 299(5615):2076-2079 (2003).
  4. Li, S., et al., Proc. Natl. Acad. Sci. U.S.A. 99(8):5567-5572 (2002).
  5. Suzuki, N., et al., Nature 416(6882):750-756 (2002).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.