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Catalog #
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Source:
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Lot #
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Size:
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AP7897b
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Rabbit
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SH090203I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001620, P20292
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RB17168
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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18157 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ALOX5AP.
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Other Names:
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Arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding protein; ALOX5AP
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Application Data:
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(LEFT)Western blot analysis of anti-AALOX5AP Antibody (C-term) (Cat.#AP7897b) in HepG2 cell line lysates (35ug/lane). AALOX5AP(arrow) was detected using the purified Pab.
(RIGHT)Western blot analysis of anti-ALOX5AP Antibody (C-term) (Cat.#AP7897b) in mouse intestine tissue lysates (35ug/lane). ALOX5AP(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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ALOX5AP is a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.
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Background References:
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- Zintzaras,E., Am. J. Epidemiol. 169 (5), 523-532 (2009)
- Crosslin,D.R., Hum. Genet. 125 (2), 217-229 (2009)
- Steinhilber,D., Pharm Acta Helv 69 (1), 3-14 (1994)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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