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Catalog #
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Source:
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Lot #
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Size:
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AP7928d
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Rabbit
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SH050104B
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_002569, O75914-2
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RB6838
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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60693 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PAK3.
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Other Names:
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Serine/threonine-protein kinase PAK 3; p21-activated kinase 3; PAK-3; Beta-PAK; Oligophrenin-3; OPHN3
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Application Data:
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Western blot analysis of PAK3 (arrow) using PAK3 Antibody (C-term) (Cat.#AP7928d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2) (Origene Technologies). |
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Background:
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PAK3, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. PAK3 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. It shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK. This protein is highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. PAK3 is autophosphorylated when activated by CDC42/p21. Defects in PAK3 are the cause of non-specific X-linked nonsyndromic mental retardation type 30 (MRX30). The protein structure contains 1 CRIB domain.
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Background References:
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- Kitano, T., et al., Mol. Biol. Evol. 20(8):1281-1289 (2003).
- Allen, K.M., et al., Nat. Genet. 20(1):25-30 (1998).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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