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Catalog #
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Source:
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Lot #
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Size:
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AP7993b
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Rabbit
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SH081230D
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000490, P04798
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RB16938
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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58165 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CYP1A1.
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Other Names:
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P450-C; P450 form 6; P450-P1; CYPIA1; Cytochrome P450 1A1; CYP1A1
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Application Data:
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(LEFT)Western blot analysis of anti-CYP1A1 Antibody (C-term) (Cat.#AP7993b) in K562 cell line lysates (35ug/lane). CYP1A1(arrow) was detected using the purified Pab.
(RIGHT)Western blot analysis of anti-CYP1A1 Antibody (C-term) (Cat.#AP7993b) in mouse lung tissue lysates (35ug/lane). CYP1A1(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP1A1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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CYP1A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. CYP1A1 gene has been associated with lung cancer risk.
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Background References:
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- Delpisheh,A., Eur. J. Obstet. Gynecol. Reprod. Biol. 143 (1), 38-42 (2009)
- Zhuo,W., Cancer Invest. 27 (1), 86-95 (2009)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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