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Catalog #
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Source:
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Lot #
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Size:
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AP8016c
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Rabbit
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SH070613P
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_006209, Q14CW1
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RB11623
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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124284 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the the center region of human PI3KCA.
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Other Names:
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Phosphatidylinositol-4;5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; PI3K; PIK3CA;
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Application Data:
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(LEFT)Western blot analysis of anti-PIK3CA Antibody (Center) Pab (Cat.#AP8016c) in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab (1:60 dilution) (RIGHT)Western blot analysis of anti-PI3KCA Antibody (Center) Pab (Cat.#AP8016c) in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center)(Cat.#AP8016c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
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Background References:
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- Broderick, D.K., et al., Cancer Res. 64(15):5048-5050 (2004).
- Ballou, L.M., et al., J. Biol. Chem. 278(26):23472-23479 (2003).
- Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
- Singh, B., et al., Genes Dev. 16(8):984-993 (2002).
- Cook, J.A., et al., J. Immunol. 169(1):254-260 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50-100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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