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Catalog #
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Source:
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Lot #
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Size:
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AP8044b
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Rabbit
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SH020802C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q9H4B4
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RB0875
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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71789 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PLK3.
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Other Names:
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Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Proliferation-related kinase; CNK; FNK; PRK
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Application Data:
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Western blot analysis of anti-CNK Pab (Cat. #AP8044b) in SK-BR3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B). CNK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
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Background:
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CNK, a member of the CDC5/Polo subfamily of Ser/Thr protein kinases, is involved in regulating M phase functions during the cell cycle. It may also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. This membrane-associated protein binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain. Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. This protein exhibits a short half-live. In cells of hematopoietic origin, CNK is strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. Cytokine and cellular adhesion trigger CNK induction. CNK is thought to be phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis The protein contains 2 POLO box domains.
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Background References:
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- Holtrich, U., et al., Oncogene 19(42):4832-4839 (2000).
- Ouyang, B., et al., Oncogene 18(44):6029-6036 (1999).
- Ouyang, B., et al., J. Biol. Chem. 272(45):28646-28651 (1997).
- Li, B., et al., J. Biol. Chem. 271(32):19402-19408 (1996).
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Product Citations:
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- Zhang, Hong, et al. J. Biol. Chem. 2004 Aug 20;279(34):35726-35734. (Applications: WB)
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Flow cytometry | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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