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Catalog #
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Source:
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Lot #
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Size:
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AP8062a
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Rabbit
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SH030530E; SH030530F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001013725, Q9P2K8
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RB3305/RB3306
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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186,838 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region with phenylalanine at position 1116 of human GCN2.
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Other Names:
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Putative eIF2 alpha kinase
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Application Data:
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Western blot analysis of GCN2 (arrow) using GCN2 Antibody (Center F1116) (Cat.#AP8062a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GCN2 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1; MIM 603907) to downregulate protein synthesis in response to varied cellular stresses (Berlanga et al., 1999 [PubMed 10504407]).[supplied by OMIM]
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Background References:
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- Blume-Jensen P, et al. Nature 2001. 411: 355.
- Cantrell D, J. Cell Sci. 2001. 114: 1439.
- Jhiang S Oncogene 2000. 19: 5590.
- Manning G, et al. Science 2002. 298: 1912.
- Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
- Robertson, S. et al. Trends Genet. 2000. 16: 368.
- Robinson D, et al. Oncogene 2000. 19: 5548.
- Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
- Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
- Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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