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GNB2L1 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP8063a Rabbit SH030530H 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_006089 RB3308 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 35076 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GNB2L1.
Other Names:
Guanine nucleotide-binding protein beta subunit-like protein, P205, Receptor of activated protein kinase C 1, RACK1, Receptor for activated C kinase
Application Data:
GNB2L1 Antibody (N-term)

(LEFT)The anti-GNB2L1 Pab (Cat. #AP8063a) is used in Western blot to detect GNB2L1 in Ramos cell lysate. (RIGHT)Western blot analysis of GNB2L1 (arrow) using GNB2L1 Antibody (N-term) (Cat.#AP8063a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GNB2L1 gene (Lane 2) (Origene Technologies).

 GNB2L1 Antibody (N-term)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Background References:
  1. Ceci, M., et al., Nature 426(6966):579-584 (2003).
  2. Usacheva, A., et al., J. Immunol. 171(6):2989-2994 (2003).
  3. Kiely, P.A., et al., J. Biol. Chem. 277(25):22581-22589 (2002).
  4. Tcherkasowa, A.E., et al., J. Immunol. 169(9):5161-5170 (2002).
  5. Liedtke, C.M., et al., J. Biol. Chem. 277(25):22925-22933 (2002).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.