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Catalog #
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Source:
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Lot #
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Size:
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AP8067a
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Rabbit
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SH030606G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q8TAS1
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RB3367
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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46546 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human KIS.
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Other Names:
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Serine/threonine-protein kinase Kist, Kinase interacting with stathmin; U2AF homology motif kinase 1; UHMK1
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Application Data:
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Western blot analysis of anti-KIS Pab (Cat. #AP8067a) in mouse heart tissue lysate. KIS (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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Upon serum stimulation, KIS, a member of the Ser/Thr protein kinase family, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. This protein, which contains 1 RRM (RNA recognition motif)domain, may be involved in trafficking and/or processing of RNA. KIS is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes.
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Background References:
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- Bieche, I., et al., Brain Res. Mol. Brain Res. 114(1):55-64 (2003).
- Boehm, M., et al., EMBO J. 21(13):3390-3401 (2002).
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Product Citations:
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- Petrovic, Vladimir, et al. J. Biol. Chem. 2008 Jan 04;283(1):453-460.
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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