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Catalog #
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Source:
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Lot #
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Size:
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AP8104a
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Rabbit
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SH071109G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_003556, O75385
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RB13052
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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112601 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ULK1.
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Other Names:
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Serine/threonine-protein kinase ULK1, Unc-51-like kinase 1, ATG1, UNC51, Unc51.1, FLJ38455; APG1; ATG1
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Application Data:
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Western blot analysis of anti-Autophagy APG1 (ULK1) Antibody (N-term) (Cat.#AP8104a) in K562 cell line lysates (35ug/lane). Autophagy APG1 (ULK1) (arrow) was detected using the purified Pab (1:60 dilution). |
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Background:
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Two human homologs of the yeast autophagy-specific kinase exist: ULK1(APG1) and ULK2. APG1 plays a critical role in regulating key elements of the autophagy pathway. APG1 stimulates autophagy, leading to autophagy-dependent restriction of cell growth and ultimately cell apoptosis at high levels of activity, and is a negative regulator of mTOR signaling.
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Background References:
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- Scott, R., et al., Current Biology 17: 1-11 (2007).
- Kuroyanagi, H., et al., Genomics 51(1):76-85 (1998).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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