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Catalog #
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Source:
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Lot #
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Size:
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AP8110a
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Rabbit
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SH030121W
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NEMO_HUMAN
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RB2163
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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48197 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length sequence of human IKK gamma.
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Other Names:
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NF-kappaB essential modulator, NEMO, NF-kappaB essential modifier, Inhibitor of nuclear factor kappa-B kinase gamma subunit, IkB kinase gamma subunit, I-kappa-B kinase gamma, IKKG, IkB kinase-associated protein 1, IKKAP1, FIP-3
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Application Data:
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The anti-IKK gamma Pab (Cat. #AP8110a) is used in Western blot to detect HL-60 cell lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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IKK gamma is a regulatory subunit part of the IKK-signalosome complex activation. It is also considered to be a mediator for TAX activation of NF-kappa-B. This protein could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Defects in IKBKG are the cause of anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID). EDA-ID is a X-linked recessive disorder characterized by absence of sweat glands, sparse scalp hair, rare conical teeth and immunological abnormalities resulting in severe infectious diseases. Defects in IKBKG are the cause of familial incontinentia pigmenti type II (IP2), an X-linked dominant disease causing death in male fetuses. In heterozygous female, it is characterized by disturbance of skin pigmentation sometimes associated with a variety of malformations of the eye, nails, teeth, skeleton, heart, and central nervous system.
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Background References:
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- Tang, E.D., et al., J. Biol. Chem. 278(39):37297-37305 (2003).
- Mauro, C., et al., Biochem. Biophys. Res. Commun. 309(1):84-90 (2003).
- Tegethoff, S., et al., Mol. Cell. Biol. 23(6):2029-2041 (2003).
- Weil, R., et al., Immunity 18(1):13-26 (2003).
- Chariot, A., et al., J. Biol. Chem. 277(40):37029-37036 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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