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Catalog #
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Source:
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Lot #
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Size:
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AP8110d
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Rabbit
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SA051222D
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_003630, Q9Y6K9
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RB6906
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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48198 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human IKK gamma.
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Other Names:
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NF-kappa-B essential modulator; NEMO; NF-kappa-B essential modifier; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IkB kinase subunit gamma; I-kappa-B kinase gamma; IKK-gamma; IKKG; IkB kinase-associated protein 1; IKKAP1; FIP-3; IKBKG; FIP3; NEMO;
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Application Data:
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(LEFT) Western blot analysis of anti-IKK gamma Antibody (N-term Q24) (Cat.#8110d) in T47D cell line lysates (35ug/lane). IKK gamma (arrow) was detected using the purified Pab.
(RIGHT) Western blot analysis of IKK gamma Antibody (N-term Q24) (Cat.# AP8110d)in human 293 cell line lysate (lane 1: raw 293 lysate; lane 2: induced by EGF 100ng/ml 30min; lane 3: induced by EGF 200ng/ml 30min). IKK gamma (arrow) was detected using the purified PAb. |
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Background:
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IKK gamma is a regulatory subunit part of the IKK-signalosome complex activation. It is also considered to be a mediator for TAX activation of NF-kappa-B. This protein could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Defects in IKBKG are the cause of anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID). EDA-ID is a X-linked recessive disorder characterized by absence of sweat glands, sparse scalp hair, rare conical teeth and immunological abnormalities resulting in severe infectious diseases. Defects in IKBKG are the cause of familial incontinentia pigmenti type II (IP2), an X-linked dominant disease causing death in male fetuses. In heterozygous female, it is characterized by disturbance of skin pigmentation sometimes associated with a variety of malformations of the eye, nails, teeth, skeleton, heart, and central nervous system.
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Background References:
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- Orange, J.S., et al., J. Allergy Clin. Immunol. 114(3):650-656 (2004).
- Stilo, R., et al., J. Biol. Chem. 279(33):34323-34331 (2004).
- Nishikomori, R., et al., Blood 103(12):4565-4572 (2004).
- Orange, J.S., et al., J. Allergy Clin. Immunol. 113(4):725-733 (2004).
- Yang, F., et al., J. Immunol. 172(4):2446-2452 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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