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Catalog #
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Source:
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Lot #
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Size:
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AP8138a
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Rabbit
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SH030813I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q14164
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RB3909
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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80462 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human IKKE.
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Other Names:
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Inhibitor of nuclear factor kappa-B kinase epsilon subunit; I kappa-B kinase epsilon; IkBKE; IKK-epsilon; IKK-E; Inducible I kappa-B kinase; IKK-i
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Application Data:
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The anti-IKKE Pab (Cat. #AP8138a) is used in Western blot to detect IKKE in THP-1 cell lysate. |
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Background:
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Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
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Background References:
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- Blume-Jensen P, et al. Nature 2001. 411: 355.
- Cantrell D, J. Cell Sci. 2001. 114: 1439.
- Jhiang S Oncogene 2000. 19: 5590.
- Manning G, et al. Science 2002. 298: 1912.
- Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
- Robertson, S. et al. Trends Genet. 2000. 16: 368.
- Robinson D, et al. Oncogene 2000. 19: 5548.
- Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
- Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
- Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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