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HK2 (Hexokinase II) Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP8140a Rabbit SH030814E 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml P52789 RB3917 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 102367 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human HK2.
Other Names:
Hexokinase, type II; HK II; Muscle form hexokinase
Application Data:
HK2 (Hexokinase II) Antibody (N-term)

The anti-HK2 Pab (Cat. #AP8140a) is used in Western blot to detect HK2 in A375 cell lysate.

 HK2 (Hexokinase II) Antibody (N-term)

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
Background References:
  1. Lehto, M., et al., Diabetologia 38(12):1466-1474 (1995).
  2. Vidal-Puig, A., et al., Diabetes 44(3):340-346 (1995).
  3. Laakso, M., et al., Diabetes 44(3):330-334 (1995).
  4. Echwald, S.M., et al., Diabetes 44(3):347-353 (1995).
  5. Shinohara, Y., et al., Cancer Lett. 82(1):27-32 (1994).
Product Citations:
  1. Diane Palmieri, et al. Mol. Cancer Res. 2009; 7:1438-1445.
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.