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Catalog #
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Source:
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Lot #
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Size:
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AP8155b
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Rabbit
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SH030903H
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P31946
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RB4064
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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27951 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human YWHAB.
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Other Names:
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14-3-3 protein beta/alpha; Protein kinase C inhibitor protein-1; KCIP-1; Protein 1054
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Application Data:
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The anti-YWHAB Pab (Cat. #AP8155b) is used in Western blot to detect YWHAB in Jurkat cell lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
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Background References:
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- Komori, T., et al., Acta Neuropathol. 106(1):66-70 (2003).
- Cavet, M.E., et al., J. Biol. Chem. 278(20):18376-18383 (2003).
- Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003).
- Shumway, S.D., et al., J. Biol. Chem. 278(4):2089-2092 (2003).
- Parvaresch, S., et al., FEBS Lett. 532(3):357-362 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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