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Catalog #
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Source:
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Lot #
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Size:
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AP8407a
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Rabbit
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SH020611P
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P29074
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RB0423
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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105911 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MEG1.
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Other Names:
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Application Data:
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The anti-MEG1 N-term Pab (Cat. #AP8407a) is used in Western blot to detect MEG1 in mouse brain tissue lysate. |
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MEG1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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MEG1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP has been shown to interact with glutamate receptor delta 2 and epsilon subunits, and is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.
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Background References:
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- Hironaka, K., et al., J. Biol. Chem. 275(21):16167-16173 (2000).
- Gu, M., et al., Proc. Natl. Acad. Sci. U.S.A. 93(23):12980-12985 (1996).
- Gu, M., et al., J. Biol. Chem. 271(44):27751-27759 (1996).
- Gu, M.X., et al., Proc. Natl. Acad. Sci. U.S.A. 88(13):5867-5871 (1991).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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