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Catalog #
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Source:
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Lot #
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Size:
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AP8409a
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Rabbit
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SH020705W
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q15256
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RB0621
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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73862 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PCPTP1.
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Other Names:
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Application Data:
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The anti-PCPTP1 Pab (Cat. #AP8409a) is used in Western blot to detect PCPTP1 in placenta tissue lysate. |
Formalin-fixed and paraffin-embedded human brain tissue reacted with hPCPTP1-Y249, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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PCPTP1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domains, and thus represents a receptor-type PTP. The similar gene for this protein predominately expressed in mouse brain was found to associate with, and thus regulate the activity and cellular localization of MAP kinases. The rat counterpart of the gene for this protein was reported to be regulated by the nerve growth factor, which suggested a function in neuronal growth and differentiation.
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Background References:
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- Blanco-Aparicio, C., et al., J. Cell Biol. 147(6):1129-1136 (1999).
- Ogata, M., et al., J. Biol. Chem. 270(5):2337-2343 (1995).
- Shiozuka, K., et al., Gene 162(2):279-284 (1995).
- Sharma, E., et al., J. Biol. Chem. 270(1):49-53 (1995).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500" |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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