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Catalog #
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Source:
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Lot #
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Size:
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AS1003a
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Rabbit
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N/A
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0.02 mg/antibody
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/mL
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N/A
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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N/A
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Target/Specificity:
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Select five or more methylation antibodies from our comprehensive product line.
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Other Names:
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Application Data:
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Western blot analysis of anti-Dnmt3a Pab (Cat. #AP1034a) in T47-D cell lysate. Dnmt3a (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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Post-translational modifications of core histones (H1, H2A, H2B, H2, and H4) such as methylation, play a pivotal role in a variety of gene activities such as transcription regulation, DNA replication and damage repair, gene silencing and the regulation of cell developmental processes such as cellular differentiation and proliferation. These modifications, clustered on the relatively highly charged N-terminal tails of histones, result in remodeling of the chromatin structure. Conformational adjustment controls accessibility to transcription complexes and thereby directs promoter-specific events as well as overall controls of chromosomal activity. Key enzymes effecting these modifications include histone methyltransferase (HMT); the proper balance of protein methylation is crucial to appropriate cellular transcription.
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Addition of a methyl group to a cytosine residue of DNA by DNA methyltransferase converts it to 5-methylcytosine. The level of methylation of CpG islands, which cluster densely near eucaryotic gene promoters, strongly determines gene activity and expression; deposition of methyl groups on DNA play a role in inducing oncogenic transcription.
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Background References:
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- Curr Med Chem. 2008;15(1):92-106.
- Curr Opin Chem Biol. 2007 Dec;11(6):628-35.
- Nat Struct Mol Biol. 2007 Nov;14(11):1008-16.
- Development. 2007 Nov;134(22):3959-65.
- Nat Rev Genet. 2007 Nov;8(11):829-33.
- Expert Rev Mol Diagn. 2007
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Product Citations:
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Application Notes:
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| Antibodies have been broadly tested in ELISA, | |
| Western blot, and IHC applications. | |
| Immunofluorescence data is also demonstrated | |
| for select products. Check individual product | |
| technical data sheets for details. | |
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Format:
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Purified polyclonal antibodies supplied in PBS with 0.09% (W/V) sodium azide. The antibodies are purified through protein G columns, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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