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Catalog #
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Source:
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Lot #
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Size:
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AT2337a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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AAH00301
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5C11
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IgG1 Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,IF,IHC-P,S-ELISA,WB-Ce,WB-Re
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H
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55103 Da
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Target/Specificity:
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HDAC1 (AAH00301, 1 a.a. ~ 483 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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DKFZp686H12203, GON10, HD1, RPD3, RPD3L1
histone deacetylase 1
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Application Data:
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Immunofluorescence of monoclonal antibody to HDAC1 (AT2337a) on HeLa cell . [antibody concentration 10 ug/ml] |
Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11.
Lane 1: HDAC1 transfected lysate(55.1 KDa).
Lane 2: Non-transfected lysate.
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Background:
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Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
| Immunohistochemistry | 1.5ug/ml |
| Immunofluorescence | |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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