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Catalog #
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Source:
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Lot #
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Size:
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AT2479a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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NP_002159
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5F11
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IgG1 Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,IHC-P,S-ELISA,WB-Re
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H
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50909 Da
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Target/Specificity:
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IDH2 (NP_002159, 354 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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ICDM, IDH, IDHM, IDP, mNADPIDH
isocitrate dehydrogenase 2 (NADP+), mitochondrial
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Application Data:
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Immunoperoxidase of monoclonal antibody to IDH2 ( Cat # AT2479a ) on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] |
Detection limit for recombinant GST tagged IDH2 is approximately 0.03ng/ml as a capture antibody. |
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Background:
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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
| Immunohistochemistry | 1.5ug/ml |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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