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Catalog #
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Source:
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Lot #
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Size:
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AT2690a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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NP_000518
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5E7
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IgG2a Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,S-ELISA
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H
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95376 Da
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Target/Specificity:
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LDLR (NP_000518, 105 a.a. ~ 206 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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FH, FHC
low density lipoprotein receptor (familial hypercholesterolemia)
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Application Data:
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Western Blot analysis of LDLR expression in transfected 293T cell line by LDLR monoclonal antibody (M01), clone 5E7.
Lane 1: LDLR transfected lysate(94.6 KDa).
Lane 2: Non-transfected lysate.
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Detection limit for recombinant GST tagged LDLR is approximately 0.3ng/ml as a capture antibody. |
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Background:
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The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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