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Catalog #
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Source:
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Lot #
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Size:
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AT2830a
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mouse
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08319
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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AAH25246
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3H5
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IgG1 Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,IHC-P,S-ELISA,WB-Ce,WB-Re
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H
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64150 Da
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Target/Specificity:
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ME1 (AAH25246, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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HUMNDME, MESmalic enzyme 1, NADP(+)-dependent, cytosolic
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Application Data:
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Western Blot analysis of ME1 expression in transfected 293T cell line by ME1 monoclonal antibody (M03), clone 3H5.
Lane 1: ME1 transfected lysate(64.1 KDa).
Lane 2: Non-transfected lysate.
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ME1 monoclonal antibody (M03), clone 3H5. Western Blot analysis of ME1 expression in HepG2 ( Cat # L019V1 ). |
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Background:
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This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
| Immunohistochemistry | 1.5ug/ml |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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