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Catalog #
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Source:
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Lot #
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Size:
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AT2895a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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AAH56865
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4D7
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IgG1 Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,RNAi-Ab,S-ELISA,WB-Re
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H
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66152 Da
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Target/Specificity:
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MPP3 (AAH56865, 1 a.a. ~ 586 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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DLG3
membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
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Application Data:
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Western blot analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi ( Cat # H00004356-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP3 monoclonal antibody (M01), clone 4D7 (Cat # AT2895a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. |
Western Blot analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody (M01), clone 4D7.
Lane 1: MPP3 transfected lysate(66.2 KDa).
Lane 2: Non-transfected lysate.
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Background:
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This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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