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Catalog #
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Source:
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Lot #
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Size:
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AT3036a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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AAH51765
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3F6
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IgG1 Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,IF,IHC-P,S-ELISA,WB-Ce,WB-Re
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H
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105356 Da
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Target/Specificity:
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NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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DKFZp686C01211, EBP1, KBF1, MGC54151, NFkappaB, NFKBp105, NFKBp50
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)
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Application Data:
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Immunofluorescence of monoclonal antibody to NFKB1 (AT3036a) on HeLa cell . [antibody concentration 10 ug/ml] |
NFKB1 monoclonal antibody (M03), clone 3F6 Western Blot analysis of NFKB1 expression in Hela NE ( Cat # L013V3 ). |
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Background:
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This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
| Immunohistochemistry | 1.5ug/ml |
| Immunofluorescence | |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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