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Catalog #
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Source:
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Lot #
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Size:
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AT3085a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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AAH02398
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3B2
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IgG1 kappa
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Applications:
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Reactivity:
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MW:
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ELISA,IF,IHC-P,WB-Ce,WB-Re
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H
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32575 Da
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Target/Specificity:
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NPM1 (AAH02398, 1 a.a. ~ 295 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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B23, NPM
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
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Application Data:
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Immunofluorescence of monoclonal antibody to NPM1 (AT3085a) on HeLa cell . [antibody concentration 10 ug/ml] |
NPM1 monoclonal antibody (M01), clone 3B2 Western Blot analysis of NPM1 expression in Hela NE ( Cat # L013V3 ). |
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Background:
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NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
| Immunohistochemistry | 1.5ug/ml |
| Immunofluorescence | |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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