|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AT3465a
|
mouse
|
|
0.1mg
|
|
Type:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
Mab
|
AAH08713
|
1B9
|
IgG1 Kappa
|
|
Applications:
|
Reactivity:
|
MW:
|
|
ELISA,WB-Ce,WB-Re
|
H
|
49204 Da
|
|
Target/Specificity:
|
|
PSMC3 (AAH08713, 53 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
|
|
Other Names:
|
|
MGC8487, TBP1
proteasome (prosome, macropain) 26S subunit, ATPase, 3
|
|
Application Data:
|
PSMC3 monoclonal antibody (M01), clone 1B9 Western Blot analysis of PSMC3 expression in HeLa ( Cat # L013V1 ). |
|
|
Background:
|
|
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq]
|
|
Background References:
|
|
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
|
|
Format:
|
|
Clear, colorless solution in phosphate buffered saline, pH 7.2 .
|
|
Storage:
|
|
For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
