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Catalog #
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Source:
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Lot #
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Size:
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AT3574a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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NP_002881
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2C12
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IgG1 kappa
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Applications:
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Reactivity:
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MW:
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ELISA,IHC-P,WB-Ce,WB-Re
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H
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116403 Da
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Target/Specificity:
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RASA1 (NP_002881, 948 a.a. ~ 1048 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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CMAVM, DKFZp434N071, GAP, PKWS, RASA, RASGAP, p120GAP
RAS p21 protein activator (GTPase activating protein) 1
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Application Data:
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RASA1 monoclonal antibody (M01), clone 2C12 Western Blot analysis of RASA1 expression in IMR-32 ( Cat # L008V1 ). |
Immunoperoxidase of monoclonal antibody to RASA1 ( Cat # AT3574a ) on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 6 ug/ml] |
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Background:
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The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
| Immunohistochemistry | 1.5ug/ml |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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