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Catalog #
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Source:
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Lot #
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Size:
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AT3767a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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NP_002958
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5A11
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IgG2b Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,IF,IHC-P,S-ELISA,WB-Ce,WB-Re
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H
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102642 Da
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Target/Specificity:
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SAFB (NP_002958, 111 a.a. ~ 201 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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DKFZp779C1727, HAP, HET, SAFB1
scaffold attachment factor B
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Application Data:
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Immunofluorescence of monoclonal antibody to SAFB (AT3767a) on HeLa cell . [antibody concentration 10 ug/ml] |
SAFB monoclonal antibody (M04), clone 5A11 Western Blot analysis of SAFB expression in Hela NE ( Cat # L013V3 ). |
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Background:
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This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
| Immunohistochemistry | 1.5ug/ml |
| Immunofluorescence | |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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