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Catalog #
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Source:
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Lot #
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Size:
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AT4136a
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mouse
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08298
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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NP_004597
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1E11
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IgG1 Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,S-ELISA,WB-Re
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H
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212677 Da
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Target/Specificity:
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TAF1 (NP_004597, 1784 a.a. ~ 1894 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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BA2R, CCG1, CCGS, NSCL2, OF, P250, TAF2A, TAFII250TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
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Application Data:
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Detection limit for recombinant GST tagged TAF1 is approximately 0.1ng/ml as a capture antibody. |
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Background:
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Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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