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Catalog #
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Source:
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Lot #
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Size:
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AT4430a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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AAH50736
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3B1
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IgG2a Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,WB-Re,WB-Tr
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H
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19652 Da
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Target/Specificity:
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UBE2C (AAH50736, 1 a.a. ~ 180 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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UBCH10, dJ447F3.2
ubiquitin-conjugating enzyme E2C
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Application Data:
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Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M04), clone 3B1.
Lane 1: UBE2C transfected lysate(19.7 KDa).
Lane 2: Non-transfected lysate.
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Background:
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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