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Catalog #
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Source:
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Lot #
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Size:
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AT4545a
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mouse
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0.1mg
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Type:
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Accession:
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Clone Name:
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Isotype:
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Mab
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NP_000369
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2H4
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IgG2b Kappa
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Applications:
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Reactivity:
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MW:
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ELISA,WB-Ce,WB-Re
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H
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49188 Da
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Target/Specificity:
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WT1 (NP_000369, 349 a.a. ~ 439 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Other Names:
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GUD, WAGR, WIT2, WT33
Wilms tumor 1
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Application Data:
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WT1 monoclonal antibody (M01), clone 2H4 Western Blot analysis of WT1 expression in A-431 ( Cat # L015V1 ). |
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Background:
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This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]
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Background References:
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Product Citations:
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Application Notes:
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| ELISA | 1ug/ml~3ng/ml |
| Western blot | 1:500~1000 |
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Format:
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Clear, colorless solution in phosphate buffered saline, pH 7.2 .
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Storage:
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For continuous use, store at 2-8°C for one-two days. For extended storage, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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