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Catalog #
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Source:
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Lot #
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Size:
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BP1038c
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In vitro synthesis
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SD050614X
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0.1 mg
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Concentration:
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Accession:
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Synthesis ID:
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Isotype:
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Powder
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O95983
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2103113
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Applications:
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Reactivity:
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MW (kDa):
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1655.73 Da
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Target/Specificity:
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The synthetic peptide sequence used to generate the antibody AP1038c was selected from the C-term region of human MBD3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
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Other Names:
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Methyl-CpG-binding Domain 3
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Application Data:
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Background:
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DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found
to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol.
MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and
believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem
cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
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Background References:
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- Bhattacharya SK, Ramchandani S, Cervoni N, Szyf. M. Nature, 397 (6720):579-583 1999.
- Hendrich B and Bird A. Mol Cell Biol, 18: 6538-6547(1998).
- Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A. J Biol Chem 275 (42): 32422-32429 (2000).
- Bader S, Walker M, Harrison D. Br J Cancer 83(12): 1646-1649 (2000).
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Product Citations:
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Application Notes:
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Format:
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The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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