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Catalog #
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Source:
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Lot #
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Size:
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BP3231a
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In vitro synthesis
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SD050730W
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0.1 mg
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Concentration:
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Accession:
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Synthesis ID:
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Isotype:
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Powder
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NP_004575, Q99638
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4101210
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Applications:
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Reactivity:
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MW (kDa):
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1728.23 Da
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Target/Specificity:
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The synthetic peptide sequence used to generate the antibody AP3231a was selected from the region of human Phospho-Rad9-Y28. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
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Other Names:
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Cell cycle checkpoint control protein; RAD9 homolog A; S. pombe; RAD9A;
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Application Data:
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Background:
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Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.
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Background References:
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- Maniwa, Y., et al., Cancer 103(1):126-132 (2005).
- Wang, W., et al., Proc. Natl. Acad. Sci. U.S.A. 101(48):16762-16767 (2004).
- Lindsey-Boltz, L.A., et al., (er) Nucleic Acids Res. 32(15):4524-4530 (2004).
- Toueille, M., et al., (er) Nucleic Acids Res. 32(11):3316-3324 (2004).
- Loegering, D., et al., J. Biol. Chem. 279(18):18641-18647 (2004).
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Product Citations:
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Application Notes:
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Format:
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The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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