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Catalog #
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Source:
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Lot #
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Size:
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SP1046a
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In vitro synthesis
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SD7080706A
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0.5 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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N/A
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NP_003757, Q14457
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7080706
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Applications:
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Reactivity:
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MW (kDa):
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H
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5069 Da
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Target/Specificity:
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A cell-permeable Antennapedia sequence (RQIKIWFQNRRMKWKK) is fused with mutant Beclin1-BH3 (Bcl-2 homology 3 domain of Beclin1). The fusion sequence is NH2-RQIKIWFQNRRMKWKK GTMENLSRRAKVTGDLFDIMSGQTDV-COOH.
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Other Names:
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Beclin 1; APG6; Coiled-coil myosin-like BCL2-interacting protein; Bcl-2-interacting protein beclin; Protein GT197; BECN1; GT197; ATG6
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Application Data:
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Background:
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 has recently been identified as novel BH3-only protein, meaning that it carries one Bcl-2-homology-3 (BH3) domain. Beclin 1 interacts with anti-apoptotic multidomain proteins of the Bcl-2 family by virtue of its BH3 domain, an amphipathic alpha-helix that binds to the hydrophobic cleft of Bcl-2/Bcl-X(L). The BH3 domains of other BH3-only proteins such as Bad, as well as BH3-mimetic compounds such as ABT737, competitively disrupt the inhibitory interaction between Beclin 1 and Bcl-2/Bcl-X(L). This causes autophagy of mitochondria (mitophagy) but not of the endoplasmic reticulum (reticulophagy).
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Background References:
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- Feng W, et al. J Mol Biol. 2007. 372(1):223.
- Erlich S, et al. Autophagy. 2007. 3(6):561.
- Maiuri MC, et al. EMBO J. 2007. 26(10):2527.
- Maiuri MC, et al. Autophagy. 2007. 3(4):374.
- Oberstein A, et al. J Biol Chem. 2007. 282(17):13123.
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Product Citations:
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Application Notes:
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Format:
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The synthetic peptide was HPLC purified, lyophilized with 100% acetonitrile and is supplied as a powder. The peptide can be reconstituted in solution using the appropriate buffer as needed.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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