SUMOplotAnalysis Program

Your protein migrates at a higher MW!
Do you know if it is sumoylated?

The SUMOplot Analysis Program predicts and scores sumoylation sites in your protein. The presence of this post-translational modification may help explain larger MWs than expected on SDS gels due to attachment of SUMO protein (11kDa) at multiple positions in your protein. This user-friendly program is free to the research community with a simple registration. Features include:

• Color-coded motifs with high vs. low probability for easy analysis
• Data displayed in both sequence and convenient table format
• Literature validated (see citations below)

Log in for access to the SUMOplot Analysis Program !

CITATIONS

Tiziana de Cristofaro, et al. Pax8 protein stability is controlled by sumoylation. J. Mol. Endocrinol., Jan 2009; 42: 35 - 46.   Abstract   

Yurchenko, Vyacheslav, et al. SUMO Modification of Human XRCC4 Regulates Its Localization and Function in DNA Double-Strand Break Repair. Mol. Cell. Biol. 2006 Mar 01;26(5):1786-1794.   Abstract   Full Article

Deng, Zhiyong, et al. PIASy-Mediated Sumoylation of Yin Yang 1 Depends on Their Interaction but Not the RING Finger. Mol. Cell. Biol. 2007 May 15;27(10):3780-3792.   Abstract   Full Article

Yang, Catherine K., et al. Role of the N-Terminal Activation Domain of the Coiled-Coil Coactivator in Mediating Transcriptional Activation by {beta}-Catenin. Mol. Endocrinol. 2006 Dec 01;20(12):3251-3262.   Abstract   Full Article

Xue, Yu, et al. SUMOsp: a web server for sumoylation site prediction. Nucleic Acids Res. 2006 Jul 01;34(suppl_2):W254-257.   Abstract   Full Article

Bish, Rebecca A., et al. Werner Helicase-interacting Protein 1 Binds Polyubiquitin via Its Zinc Finger Domain. J. Biol. Chem. 2007 Aug 10;282(32):23184-23193.   Abstract   Full Article

Rytinki, Miia M., et al. Sumoylation modulates the transcription repressor function of receptor interacting protein 140. J. Biol. Chem. 2008 Apr 25;283(17):11586-95.   Abstract   Full Article

Matarasso, Noa, et al. A Novel Plant Cysteine Protease Has a Dual Function as a Regulator of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene Expression. PLANT CELL 2005 Apr 01;17(4):1205-1216.   Abstract   Full Article

Miura, Kenji, et al. SIZ1-Mediated Sumoylation of ICE1 Controls CBF3/DREB1A Expression and Freezing Tolerance in Arabidopsis. PLANT CELL 2007 Apr 01;19(4):1403-1414.   Abstract   Full Article

Colby, Thomas, et al. SUMO-Conjugating and SUMO-Deconjugating Enzymes from Arabidopsis. Plant Physiology 2006 Sep 01;142(1):318-332.   Abstract   Full Article

Perry, Jason, et al. Bioinformatic analyses implicate the collaborating meiotic crossover/chiasma proteins Zip2, Zip3, and Spo22/Zip4 in ubiquitin labeling. PNAS 2005 Dec 06;102(49):17594-17599.   Abstract   Full Article

Hinsley, Timothy A., et al. Comparison of TFII-I gene family members deleted in Williams-Beuren syndrome. Protein Sci. 2004 Oct 01;13(10):2588-2599.   Abstract   Full Article

Man, Jiang-Hong, et al. PIAS3 induction of PRB sumoylation represses PRB transactivation by destabilizing its retention in the nucleus. Nucleic Acids Res. 2006 Nov 14;34(19):5552-5566.   Abstract   Full Article

Van Dyck, Frederik, et al. Repression of the Transactivating Capacity of the Oncoprotein PLAG1 by SUMOylation. J. Biol. Chem. 2004 Aug 20;279(34):36121-36131.   Abstract   Full Article

Tang, Zhongshu, et al. Pias1 Interaction and Sumoylation of Metabotropic Glutamate Receptor 8. J. Biol. Chem. 2005 Nov 18;280(46):38153-38159.   Abstract   Full Article

Klaus Schwamborn, et al. SUMO Assay with Peptide Arrays on Solid Support: Insights into SUMO Target Sites. J. Biochem., Jul 2008; 144: 39 - 49.   Abstract   

Hsu-Wen Chao, et al. SUMOylation of the MAGUK protein CASK regulates dendritic spinogenesis. J. Cell Biol., Jul 2008; 182: 141 - 155.   Abstract   

Wang, Qi-En, et al. Ubiquitylation-independent degradation of Xeroderma pigmentosum group C protein is required for efficient nucleotide excision repair. Nucleic Acids Res. 2007 Aug 13;35(16):5338-5350.   Abstract   Full Article

Abdulsamie Hanano, et al. Plant seed peroxygenase is an original heme-oxygenase with an EF-hand calcium-binding motif. J Biol Chem. 2006 Nov 3;281(44):33140-51.   Abstract   Full Article

Domanskyi A, et al. Biochemical characterization of androgen receptor-interacting protein 4. Biochem J. 2006 Feb 1;393(Pt 3):789-95.   Abstract   Full Article

Nida Meednu, et al. The Spindle Positioning Protein Kar9p Interacts with the Sumoylation Machinery in Saccharomyces cerevisiae. Genetics, Dec 2008; 180: 2033 - 2055.   Abstract   Full Article

Petrice W. Brown, et al. Small ubiquitin-related modifier (SUMO)-1, SUMO-2/3 and SUMOylation are involved with centromeric heterochromatin of chromosomes 9 and 1 and proteins of the synaptonemal complex during meiosis in men. Hum. Reprod., Dec 2008; 23: 2850 - 2857.   Abstract   Full Article

Ruchika Budhiraja, et al. Substrates Related to Chromatin and to RNA-Dependent Processes Are Modified by Arabidopsis SUMO Isoforms that Differ in a Conserved Residue with Influence on De-Sumoylation. Plant Physiology, Jan 2009; 10.1104/pp.108.135053. (E-pub)   Abstract   Full Article

Kenji Miura, Jiyoung Lee, et al. Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling. PNAS. 2009; 106:5418-5423. (E-pub)   Abstract   

Sachiko Okada, et al. Reconstitution of Arabidopsis thaliana SUMO Pathways in E. coli: Functional Evaluation of SUMO Machinery Proteins and Mapping of SUMOylation Sites by Mass Spectrometry. Plant Cell Physiol. 2009; 50:1049-1061.   Abstract