Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Antibody Validation ServicesLet Us Be Your Lab Certify that your antibodies work, while saving time and money. Abgent’s experienced staff custom validates more than 1,000 antibodies each month in Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC) and additional applications. Proper validation with controls and optimization ensure the quality of the antibody, and establish confidence with the researcher.
Abgent customers include academic and biotech labs, as well as the largest antibody companies in the world. We make validation easy and affordable for you. It is what we do, day in and day out, with excellence. Let Us Be Your Lab |
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Immunofluorescence (IF)Fluorescent image of Hela cells stained with PPARA Antibody. This antibody was diluted at 1:25. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). |
ImmImmunohistochemistry (IHC)Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using PPARA Antibody. This antibody was diluted at 1:25. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. |
Flow Cytometric (FC)Flow cytometric analysis of Hela cells using PPARA Antibody (green), compared to a isotype control of mouse IgG1(blue). This antibody was diluted at 1:25. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody. |
Western Blot (WB)Western blot analysis of lysates from Hela, Jurkat, mouse NIH/3T3 cell line (from left to right), using PPARA Antibody. This antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates are loaded at 35ug per lane. |
Abgent's Antibody Validation Management System "SuperView".
Login from anywhere in the world and manage your custom antibody validation projects at your convenience. Learn More
